With over 99 percent of the voting districts counted in the election, ANO had garnered 34.6 percent of the vote, while the ruling center-right SPOLU coalition came in second place with 23.3 percent, Xinhua reported.
Other parties clearing the 5 percent threshold included the Mayors and Independents (STAN) with 11.2 percent, the Pirate Party with 8.9 percent, the far-right Freedom and Direct Democracy (SPD) with 7.8 percent, and the populist Motorists for Themselves with nearly 6.8 percent.
Babis called the election result the absolute highlight of his political career.
"We are extremely happy and we thank everyone who came to the polls," he said, claiming that ANO achieved a historic result.
Babis added that his party has a clear program and will change the country for the better.
"A government will be formed that will really work hard, that someone will manage and that will fulfill the program from the first moment," he said.