France's national rail company SNCF says a third of the country's high speed trains are being canceled.
Eurostar trains to London and Thalys trains to Brussels are expected to run normally Wednesday.
In Paris, only half of suburban trains are circulating on certain lines. Paris subway is unaffected by the strike, AP reported.
Rail unions are protesting a plan to change working hours and rules about overtime and days off. They are also rejecting a contested labor law aiming at loosening the country's labor rules.
Some truck drivers were also continuing a strike that started Tuesday, blocking roads around cities in western France.